![]() ![]() ![]() Isotope ratios of materials routinely range over 4-8 orders of magnitude in a single experiment, dosing solutions contain thousands of DPM and gels used to separate proteins possess 14 C ratios of 1pMC. Maintaining a contamination free sample preparation lab for biological 14 C AMS requires the same or more diligence as a radiocarbon dating prep lab. Tips and traps in the 14C Bio-AMS preparation laboratory (WSam 7)Ä«uchholz, B A Haack, K W Stewart, P H Vogel, J S Cross-contamination of samples can be minimized by using disposable plastic or glassware in the prep lab, isolating samples from the air when possible and using positive displacement pipettors Frequent air changes help minimize aerosol contamination in many cases. The monitors can be set out in work spaces for 1-2 weeks and measured by AMS with regular samples. Aerosols can be monitored for 14 C content using graphitized coal or fullerene soot mixed with metal powder as an absorber. Shared air supplies can also present problems by distributing hot aerosols throughout a building. In general, all surfaces routinely touched need to be covered with paper, foil or plastic that can be changed frequently. ![]() Contamination can be found on any surface routinely touched: door knobs, light switches, drawer handles, water faucets. Removable surface contamination can be found and monitored using swipes. Radiocarbon contamination is a legacy of earlier tracer work in most biological laboratories, even if they were never hot labs. ![]() Isotope ratios of materials routinely range over 4-8 orders of magnitude in a single experiment, dosing solutions contain thousands of DPM and gels used to separate proteins possess 14 C ratios of 1 amol 14 C/mg C. International Nuclear Information System (INIS)Ä«uchholz, Bruce A. Tips and traps in the 14C bio-AMS preparation laboratory ![]()
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